耳聋基因治疗，人工耳蜗开发与植入后语训研究

现在承担和参与的课题：

1. 上海市教委，曙光学者计划，基于CRISPR/Cas9 定点修复 Klhl18 耳聋基因治疗，2021-01至2022-12，15万，在研，主持；

2. 科技部，国家重点研发项目，2020YFA0908201，具有特定合成功能的生物体单元构建（课题一），2020-11-2025-10，458万，在研，主持；

3. 上海申康医院发展中心 ，临床三年行动计划重大临床研究项目，SHDC2020CR4083，遗传性耳聋智能化诊断与推广，2020-10至2022-09，50万，在研，主持；

4. 复旦大学，双优双值项目-“优秀医师-优秀临床研究者”计划（双优计划），SYB202005，中国耳硬化临床流行病学多中心调查研究，2020-01至2022-12，20万，在研，主持；

5. 复旦大学，“卓越2025”卓识人才项目，JIF158603，遗传性耳聋基因编辑与基因治疗，2019-12至2022-12，在研，200万，主持；

6. 国家自然科学基金，优秀青年项目，81822011，感音神经性耳聋干预研究，2019-01至2021-12，130万，在研，主持；

7. 国家自然科学基金，面上项目，81771013，基于CRISPR/Cas9定点编辑蛋白系统对Myo6突变遗传性耳聋的基因治疗研究，2018-01至2021-12，56万，在研，主持；

8. 上海市科委，政府间国际科技合作项目（芬兰），18410712400， CRISPR/Cas9编辑系统对遗传性耳聋的基因治疗，2018-04至2021-03，50万，在研，主持;

9. 上海市科委，自然科学基金探索类项目，17ZR1448600，Myo6突变遗传性耳聋模型建立和基于CRISPR/Cas9蛋白系统基因治疗的研究，2017-5至2020-4，20万，在研，主持；

10. 上海市徐汇科委，科普创新项目，儿童分泌性中耳炎所致耳聋的科普防治，2017-07至2019-04，2万，在研，主持。

1. Wang, B.#; Xin, T.#; Shen, L.; Zhang, K.; Zhang, D.; Zhang, H.; Liu, J.; Chen, B.*; Cui, W.*; Shu, Y.*, Acoustic transmitted electrospun fibrous membranes for tympanic membrane regeneration. Chemical Engineering Journal 2021, 419.

2. Gu, X.#; Wang, D. #; Xu, Z. #; Wang, J.; Guo, L.; Chai, R.; Li, G.; Shu, Y. *; Li, H. *, Prevention of acquired sensorineural hearing loss in mice by in vivo Htra2 gene editing. Genome Biology 2021, 22 (1), 86.

3. Xue Yuanyuan#, Hu Xinde#, Wang Daqi#, Li Di#, Li Yige, Wang Fang, Huang Mingqian, Gu Xi, Xu Zhijiao, Zhou Jian, Wang Jinghan, Chai Renjie, Chen Zheng-Yi, Li Geng-lin, Yang Hui, Li Huawei*, Zuo Erwei*, Shu Yilai*. Gene editing of the Myo6 semi-dominant mutation restores hearing loss in mice. Molecular Therapy,  Accepted，2021

4. Zhang, K.; Fang, Y.; Wang, B.; Cheng, X.; Chen, B. *; Shu, Y*, Frequency-specific hearing results after stapes surgery for Chinese population otosclerosis with different degrees of hearing loss. Acta Otolaryngol 2020, 140 (5), 356-360.

5. Wenwei Luo, Jingfang Wu, Kevin A. Peng , Qingzhong Li, Qiang Du,Jianghong Xu, Chunfu Dai, Fanglu Chi*, Yilai Shu*，Clinical characteristics of patients with papilloma in the external auditory canal，Laryngoscope，27-Aug-2020， 2020 Oct 8. doi: 10.1002/lary.29113

6. Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR*. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nature Biotechnology. 2015 Jan;33(1):73-80.

7. Gao X, Tao Y, Lamas V, Huang M, Yeh WH, Pan B, Hu YJ, Hu JH, Thompson DB, Shu Y, Li Y, Wang H, Yang S, Xu Q, Polley DB, Liberman MC, Kong WJ, Holt JR, Chen ZY*, Liu DR*. Treatment of autosomal dominant hearing loss by in vivo delivery of genome editing agents. Nature. 2018 Jan 11;553(7687):217-221.

8. Cui, C.#;  Yao, Q.#;  Zhang, D.#;  Zhao, Y.#;  Zhang, K.;  Nisenbaum, E.;  Cao, P.;  Zhao, K.;  Huang, X.;  Leng, D.;  Liu, C.;  Li, N.;  Luo, Y.;  Chen, B.;  Casiano, R.;  Weed, D.;  Sargi, Z.;  Telischi, F.;  Lu, H.;  Denneny, J. C., 3rd;  Shu, Y.*; Liu, X.*,Approaching Otolaryngology Patients During the COVID-19 Pandemic. Otolaryngol Head Neck Surg 2020, 163 (1), 121-131.

9. Cheng, X.;  Liu, J.;  Li, N.;  Nisenbaum, E.;  Sun, Q.;  Chen, B.;  Casiano, R.;  Weed, D.;  Telischi, F.;  Denneny, J. C., 3rd;  Liu, X. *; Shu, Y*., Otolaryngology Providers Must Be Alert for Patients with Mild and Asymptomatic COVID-19. Otolaryngol Head Neck Surg 2020, 162 (6), 809-810.

10. Kun Zhang #, Xiaoting Cheng #, Liping Zhao #, Mingqian Huang, Yong Tao, Hongbo Zhang, Jessica M. Rosenholm, Min Zhuang, Zheng-Yi Chen, Bing Chen*, Yilai Shu*. Direct Functional Protein Delivery with a Peptide into Neonatal and Adult Mammalian Inner Ear In Vivo[J].Molecular Therapy -Methods&ClinicalDevelopment,2020,18:511-519.doi.org/10.1016/j.omtm.2020.06.023.

11. Yilai Shu#, Wenyan Li#, Mingqian Huang#, Yi-Zhou Quan#, Deborah Scheffer, Chunjie Tian, Yong Tao, Xuezhong Liu,Konrad Hochedlinger, Artur A. Indzhykulian, Zhengmin Wang, Huawei Li, and Zheng-Yi Chen*, Renewed proliferation in adult mouse cochlea and regeneration of hair cells. Nature Communications 2019, 10 (1), 5530，DOI: 10.1038/s41467-019-13157-7.

12. Yilai Shu#, Lijun Wang#, Xiaoting Cheng, Chayada Tangshewinsirikul, Weili Shi, Yasheng Yuan, Zhiqiang Yang, Huawei Li, Jun Shen*, Bing Chen*, Weiguo Zou* The p.(Pro170Leu) Variant in NOG Impairs Noggin Secretion and Causes Autosomal Dominant Congenital Conductive Hearing Loss Due to Stapes Ankylosis, Journal of Genetics and Genomics, 2019 Sep 20;46(9):445-449.

13. Xinde Hu#, Jinghan Wang#, Xuan Yao#, Qinqquan Xiao#, Yuanyuan Xue, Xiaowen Shen1, Yiwen Zhang, Yanli Lu, Linyu Shi, Yilai Shu*, Huawei Li*, Hui Yang*. Screened AAV Variants Permit Efficient Transduction Access to Supporting Cells and Hair Cells. Cell Discovery.

14. Jinghan Wang#, Jun Shen#, Luo Guo, Cheng Cheng, Renjie Chai, Yilai Shu* Huawei Li* A humanized mouse,demonstrated that apparently autosomal dominant deafness caused by MYO6 p.C442Y, is inherited in a semi-dominant pattern.Hearing research, 2019 Aug;379:79-88. doi: 10.1016/j.heares.2019.04.014. Epub 2019 Apr 26.

15. Xi Gu#, Renjie Chai, Luo Guo, Biao Dong, Wenyan Li, Yilai Shu*, Xinsheng Huang1* Huawei Li*. Transduction of Adeno-Associated Virus Vectors Targeting Hair Cells and Supporting Cells in the Neonatal Mouse Cochlea. Frontiers in Cellular Neuroscience. 2019. doi: 10.3389/fncel.2019.00008.